Abstract 1621 In vitro Phosphomimetics of Targeted Residues in Saccharomyces cerevisiae Malate Dehydrogenase

Malate dehydrogenase, or MDH, is an enzyme responsible for the conversion of malate to oxaloacetate in the presence of NAD+ in the final step of the Krebs cycle. MDH, like many other metabolic enzymes, may be regulated through phosphorylation and dephosphorylation events; however, the precise residues that are the targets of these events as well as their timing with respect to the overall cellular environment remain poorly understood. A convenient method to explore these questions is to emulate continuous phosphorylation in an in vitro setting, a process called phosphomimetics.