Abstract 2351 Kinetic and Thermodynamic Investigation of Glu13 and His40 as a catalytic dyad in F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium tuberculosis

F420-dependent glucose-6-phosphate dehydrogenase (FGD) is an enzyme found in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis disease. FGD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconolactone, utilizing F420 cofactor as a hydride acceptor. This reaction is important in the production of the reduced F420 cofactor and nucleic acid biosynthesis within Mtb, as well as the development of pharmaceuticals to treat multi-drug resistant and extreme drug-resistant forms of tuberculosis.