Abstract 2593 Unraveling the cooperative dynamics of PhzS and PhzM: Understanding the ordered steps in pyocyanin biosynthesis by Pseudomonas aeruginosa

Pseudomonas aeruginosa, a Gram-negative pathogen, is a major cause of hospital-acquired infections, particularly in immunocompromised individuals. A key factor in its ability to cause disease is the production of pyocyanin, a toxic molecule that damages tissues, suppresses immune responses, and aids in biofilm formation. Despite its importance, the sequence of steps by which pyocyanin is synthesized remains unclear, particularly how the two final enzymes, PhzM and PhzS, work together. PhzM adds a methyl group to the precursor molecule phenazine-1-carboxylic acid (PCA), while PhzS adds a hydroxyl group.