Abstract 2290 Optimization of a colorimetric assay of amylase activity

Starch is one of the most widely used natural products, with a wide variety of industrial and manufacturing uses as a food additive, adhesive, textile finishing agent, and more. To the plant, it is the primary form of energy storage. During the day, starch is synthesized in chloroplasts and then broken down during the night to generate usable sugar in the form of maltose. Among the enzymes that catalyze this degradation are β-amylases, which cleave two glucose units from the non-reducing end of a polyglucan chain, forming β-maltose.

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Publication Details

Journal
Journal of Biological Chemistry
Published
2025-05-01
DOI
https://doi.org/10.1016/j.jbc.2025.109185
Primary Topic
Biological Research and Disease Studies
Type
article
Field-Weighted Citation Impact
0.00

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article

Abstract 2290 Optimization of a colorimetric assay of amylase activity

Christopher Berndsen, Jonathan D. Monroe, S. Scanlan
Journal of Biological Chemistry
Biological Research and Disease Studies
article

Abstract 2290 Optimization of a colorimetric assay of amylase activity

Christopher Berndsen, Jonathan D. Monroe, S. Scanlan
article en

Abstract

Starch is one of the most widely used natural products, with a wide variety of industrial and manufacturing uses as a food additive, adhesive, textile finishing agent, and more. To the plant, it is the primary form of energy storage. During the day, starch is synthesized in chloroplasts and then broken down during the night to generate usable sugar in the form of maltose. Among the enzymes that catalyze this degradation are β-amylases, which cleave two glucose units from the non-reducing end of a polyglucan chain, forming β-maltose.

Journal of Biological ChemistryVol. 301(5)
University of Utah (US)
National Institutes of Health, National Institute of General Medical Sciences
Openalex Percentile: Top 88%
Biological Research and Disease Studies
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