Abstract 1710 Utilizing Proximity Labeling to Identify Transient Protein Interactions with ATP-dependent Chromatin Remodeler PICKLE in Arabidopsis thaliana

Chromodomain helicase DNA-binding (CHD) proteins are a subfamily of ATP-dependent chromatin remodelers that contribute to chromatin homeostasis via a number of activities including nucleosome assembly. In plants, PICKLE (PKL) is a member of subfamily II of CHD remodelers and contributes to homeostasis of trimethylation of lysine 27 on histone 3 (H3K27me3), an epigenetic mark that represses gene expression. A phenotypic hallmark of mutant plants that lack PKL is the expression of embryonic traits in the primary root, referred to as the “pickle root” phenotype, which is linked to altered H3K27me3 homeostasis.