Abstract 1979 Late-Stage Functionalization and Utilization of Minimalist Tetrazine-Carbohydrate Probes for Real-Time, Live Cell Labeling

Glycobiology probes that can perform real-time labeling are highly sought-after tools for investigating different aspects of human and bacterial biology, including cancer research, immunology, and medicinal chemistry. A specific subset of such probes uses carbohydrates with bioorthogonal functionality to undergo rapid ligation in vivo. Previous carbohydrate- probes utilize copper-catalyzed azide-alkyne click (CuAAC) chemistry handles, and while there is a high rate of incorporation into the intracellular and extracellular space, it suffers from modest labeling rates, and the cytotoxicity of copper limits the use in live cells.