Abstract 1865 Design of a Novel Bioluminescent Assay for Histone Acetyltransferase Activity and Discovery of the Novel Protein Post-Translational Modification, Lysine Isobutyrylation

Protein acetylation and short chain acylations are critical post-translational modifications (PTMs) widespread across the proteome of eukaryotes and prokaryotes. Histone acetyltransferase (HAT) enzymes are responsible for the catalytic addition of short-chain acyl groups to lysine residues on cellular proteins using several different acyl-coenzyme A molecules as cosubstrates for the reaction. HAT enzymes regulate important DNA-templated processes such as DNA replication, gene transcription, and chromatin assembly by depositing different acyl moieties to specific lysine residues of nucleosomal histones.