Abstract 1940 An Unbiased Mutational Assessment of NAT10 reveals its Non-Canonical Function as the Driver of Carcinogenesis

N-acetyltransferase 10 (NAT10) is an essential protein that is overexpressed in many cancer types. NAT10 structure consists of two catalytic domains, namely RNA helicase, which unwinds RNA secondary structures, and GCN5-related N-acetyltransferase (GNAT) domain, which acetylates RNA and proteins. Additionally, NAT10 also has two non-catalytic domains, such as the tRNA binding domain and the domain of the unknown function (DUF1726). Mechanistically, NAT10 catalyzes the addition of acetyl-CoA to RNAs leading to the formation of N4-acetylcytidine (ac4C).