Abstract 1503 Hho1 as a posttranslational modification (PTM) regulator: Identifying protein-protein interactions between histone H3 and the Saccharomyces cerevisiae linker histone, Hho1

This research aims to enhance our understanding of Hho1 as a transcriptional regulator via direct protein-protein interaction with histone H3 (H3), detailing the contacts made between these two proteins. Current literature suggests that Hho1, and its homologs, play a critical role in gene repression and that Hho1 makes direct contact with H3 during this process. In order to further investigate how linker histone - H3 interactions regulate chromosomal dynamics, we employed the artificial crosslinking amino acid, p-benzoylphenylalanine (pBPA), which was installed through site-specific genetic encoding, to scan for H3 N-terminal tail - Hho1 binding.