Abstract 2141 Structural and functional explorations of the DNA-binding domains of the human transcription repressor capicua
The transcriptional repressor capicua (CIC) is a key protein in cellular and developmental processes, and whose mutation or improper regulation is associated with tumor progression and cancer metastasis. Previous research has shown that two subdomains of CIC, an HMG-box and a C1 domain, are required for physiological binding to an octameric consensus sequence, 5'-TGAATGAA-3'. Here, we report the crystal structure of the two CIC domains, connected by a minimal linker region, in complex with a consensus DNA oligomer in a 2.95-Å resolution crystal structure.
Authors
- Daniel Dowling (ORCID: https://orcid.org/0000-0002-2536-0905)
- Alexey Veraksa (ORCID: https://orcid.org/0000-0003-2920-080X)
- Gerardo Jiménez (ORCID: https://orcid.org/0000-0003-2765-9289)
- Jonathan K. Webb (ORCID: https://orcid.org/0000-0003-4822-6829)
- Khandan Ilkhani (ORCID: https://orcid.org/0000-0002-4697-608X)
- Marta Forés (ORCID: https://orcid.org/0000-0003-1106-8193)
- Andrew Gnann (ORCID: https://orcid.org/0000-0002-1404-5459)
- Jeremy Liew (ORCID: https://orcid.org/0000-0002-9190-8772)
Institutions
- University of Massachusetts Boston (US)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.jbc.2025.109279
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health