Abstract 2343 Functional Characterization and Inhibitor Screening of the Monotopic Phosphoglycosyl Transferase Superfamily Using a Novel Protein-Purification-Free Approach for Antibiotic Drug Discovery

The rise of antibiotic resistance has intensified the need for new bacterial targets for therapeutic intervention. Monotopic phosphoglycosyl transferases (monoPGTs), a class of exclusively prokaryotic membrane proteins that remain poorly understood, represent promising targets for pharmacological intervention against human pathogens, including Campylobacter jejuni and Streptococcus pneumoniae. MonoPGTs play a key role in glycoconjugate synthesis, which is crucial for bacterial pathogenesis. These enzymes initiate glycan biosynthesis by transferring a sugar-phosphate from a soluble nucleotide diphosphate sugar to a polyprenol phosphate acceptor in the membrane.