Abstract 2338 GJA1-20k Directly Binds Actin to Promote Protective Mitochondrial Actin Cages

GJA1-20k, an internally translated isoform of the gap junction protein connexin 43, is stress-responsive and reduces ischemia-reperfusion injury. GJA1-20k localizes to the outer membrane of mitochondria and recruits actin to trigger an actin-mediated mitochondrial protection, which results in smaller mitochondria and reduced mitochondrial oxidative damage. However, it is not known is the mechanism by which GJA1-20k and actin work towards mitochondrial protection. Here, we hypothesize that GJA1-20k promotes mitochondrial actin cages that prevent pathological mitochondrial swelling.