Abstract 2878 Molecular interactions underlying the assembly of an ESCRT complex required for the regulation of cytokinetic abscission

Melanoma-associated Antigen (MAGE) proteins are the largest group of cancer-testis antigens (CTAs).They are further divided into Type I and Type II MAGEs based on expression pattern.While Type I MAGEs are true CTAs, Type II MAGEs are ubiquitously expressed in all tissues, with a notable higher expression in brain tissue.However, the role of Type II MAGEs in normal brain physiology and brain tumorigenesis remains unknown.In this study, we aim to understand the biochemical function of Type II MAGEs in normal brain tissue to explore their potential implications in brain cancer.We used the TCGA database and XENA browser to determine the expression profile of Type II MAGEs in normal brain tissue and compare it to brain cancer tissue samples.By comparing expression data between multiple cancer types, we were able to discover similarities in Type II MAGE expression.The biochemical function of some MAGE proteins is to bind to and enhance the activity of their respective E3 Ubiquitin ligase binding partner.Our analysis revealed that MAGE D1 exhibits significantly elevated expression in brain cancer tissues compared to normal brain tissues.Also, Type II MAGEs display consistent expression patterns across multiple cancer types, suggesting a broader role in cancer.