Abstract 2475 Exploring Alternative Dyes for Enhanced Sensitivity and Specificity in the Bradford Protein Assay

The Bradford protein assay is a widely used technique for protein quantification that is based on the binding of a specific dye to protein, leading to a color change measurable at a wavelength of 595 nm. This study tested the efficacy of various dyes as substitutes for Coomassie Brilliant Blue G-250, the standard dye used in the assay. The dyes evaluated included Rose Bengal, Methyl Orange, and Carmine, among others. Each was selected for its distinct structural and spectral properties in the presence and absence of protein.