Chromosome-level genome assembly of the aphid Megoura crassicauda

Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. By integrating PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C scaffolding techniques, we constructed a genome assembly spanning 424.45 Mb genome. Approximately 93.74% of the assembly was successfully anchored into five scaffolds, with contig and scaffold N50 reaching 5.55 Mb and 103.55 Mb, respectively. The genome completeness, as evaluated by BUSCO, achieved a completeness score of 97.75%. Additionally, a total of 14,717 protein-coding genes were identified. This high-quality genome assembly of M. crassicauda serves as a valuable genomic resource, facilitating further studies into the ecological adaptations of M. crassicauda and the development of pest control strategies.

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Publication Details

Journal
Scientific Data
Published
2025-06-13
DOI
https://doi.org/10.1038/s41597-025-05328-7
Citations
1
Primary Topic
Insect-Plant Interactions and Control
Type
article
Field-Weighted Citation Impact
1.67

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article

Chromosome-level genome assembly of the aphid Megoura crassicauda

Xu Cheng, Bing Wang, Tianyu Huang, Guirong Wang
1 citations
Scientific Data
Insect-Plant Interactions and Control
1.67
article

Chromosome-level genome assembly of the aphid Megoura crassicauda

Xu Cheng, Bing Wang, Tianyu Huang, Guirong Wang
article en
1 citations

Abstract

Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. By integrating PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C scaffolding techniques, we constructed a genome assembly spanning 424.45 Mb genome. Approximately 93.74% of the assembly was successfully anchored into five scaffolds, with contig and scaffold N50 reaching 5.55 Mb and 103.55 Mb, respectively. The genome completeness, as evaluated by BUSCO, achieved a completeness score of 97.75%. Additionally, a total of 14,717 protein-coding genes were identified. This high-quality genome assembly of M. crassicauda serves as a valuable genomic resource, facilitating further studies into the ecological adaptations of M. crassicauda and the development of pest control strategies.

Scientific DataVol. 12(1)
Agricultural Genomics Institute at Shenzhen (CN), Institute of Plant Protection (CN), Chinese Academy of Agricultural Sciences (CN)
National Natural Science Foundation of China, China Postdoctoral Science Foundation, Chinese Academy of Agricultural Sciences
Openalex Percentile: Top 18%
Insect-Plant Interactions and Control
1.67
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Chromosome-level genome assembly of the aphid Megoura crassicauda — Xu Cheng, Bing Wang, et al. · Scientific Data (2025) | TGRS Research Map | TGRS