Effect of Dietary Supplementation with Bee Pollen and Ginseng on Nutrient Utilization, Immunological and Biochemical Blood Parameters and Bacterial Count in Caecum of Growing Rabbits.

This study aimed to evaluate the effects of bee pollen and ginseng as natural growth promoters on nutrient utilization, blood parameters, and bacterial count in cecum in growing Californian rabbits. Sixty rabbits (5 weeks old, avg. 642.67±15.72 g) were randomly divided into four groups: a control group on a basal diet, a ginseng group (250 mg/kg diet), a bee pollen group (250 mg/kg diet), and a combination group (250 mg ginseng + 250 mg bee pollen/kg diet). The trial terminated from 5 to 13 weeks of age. Rabbits receiving bee pollen showed significantly improved crude protein digestibility, while ginseng improved crude fiber digestion (P<0.01). No significant changes were noted in dry matter, ether extract, or total digestible nutrients. Hematological parameters such as hemoglobin, platelet count, lymphocytes, MCV, and MCHC improved significantly (P<0.05 or P<0.01) with supplementation. Biochemical profiles showed increased total protein, albumin, and AST, alongside reduced ALT, urea-N, and creatinine in all supplemented groups, with values remaining within normal physiological limits. No significant effects were observed on globulin, A/G ratio, MDA, or TAC. However, bacterial counts, including Salmonella and E. coli in the cecum, were significantly reduced (P<0.01) in supplemented groups. In conclusion, combining 250 mg/kg ginseng and bee pollen enhanced growth, immunity, nutrient utilization, and blood biochemistry in growing rabbits, suggesting a promising natural dietary supplement under hot climate conditions. Further studies are recommended with larger sample sizes and varied dosages.