CD49 in Optimization the Cologenicity of Mesenchymal Stem Cell Culture Passage_

Background: Stem Cell Populations Expressing Integrin a6 (CD49f) expression has been founded in bone mar-row-derived mesenchymal stem cells (BM-MSCs), fe-tal urinary bladder obtained MSCs, and UCB-MSCs. It is viable that the expression of CD49f might affect the stemness of MSC culture. The goal of this work was to assess the best passage of MSC culture by CD 49 surface marker. Methods: This comparative study was performed on 15 umbilical cords (UC) collected at Gynecology and Ob-stetrics Department of Sohag University Hospitals, fresh samples was taken immediately after caesarean section, maternal age within 20-36 years old, newborn weight >1300:3500 gm. The UC is gathered and kept in a sterile specimen cup comprising 0.9% normal saline at 4C° till processing (for best result within 12 –24 h of birth). Results: CD49F and CD 73 were significantly increased in Day 12 than Days 3,6, and 9. There was a significant relation between CD73 and blood grouping at 6 days. There was no significant relation between CD 49F and CD73 and fetal sex. There was a notable negative cor-relation between CD 49F and the time with maternal age at 9 days. There was no significant correlation between CD49F and CD73 and time with GA. There was no signif-icant correlation between CD49F and time with last delivery. a significant positive association existed between CD 73 and time with delivery at 6 days.Conclusion: Blood group and maternal age may influ-ence MSC culture outcomes, with time-dependent varia-tions in surface marker expression.