Investigation of anti-inflammatory and Anti-oxidant effects of physiologically attainable Quercetin concentrations through in-silico studies by Ficus religiosa seeds Extract

Background: Peepal (Ficus religiosa) has been well recognised in traditional medicine because of the involvement of therapeutic properties in Ayurvedic medicine known as ‘World Tree’ or the ‘Tree of Life'. Its seeds are believed to purify blood and promote overall health. Quercetin, a flavonoids present in the seeds, is known for its antioxidant and anti-inflammatory properties. Aim: This study presented the extraction and bioactive evaluation of the seeds of Ficus religiosa in relation to their biological activity, and focuses on the antioxidant and anti-inflammatory activity contributing to blood purification. Method: The bioactive compounds isolated from seaweeds were treated using in silico data (molecular docking and some computer models) to predict their interactions with significant enzymes and proteins related with oxidative stress and inflammation. The antioxidant activity was determined according to the ability of the compounds to remove free radicals, seeds of Ficus religiosa was extracted by Maceration and Soxhlation using Ethanol and Water as solvents. The extracts of the seeds were tested for the presence of phytochemicals through phytochemical screening. The Blood Purification of the seeds were evaluated by using Analytical methods HPTLC (High Performance Thin Layer Chromatography for the presences of Quercetin Results: As evident from the experimental data the ethanol extract showed the presence of Quercetin through HPTLC (High Performance or Presuure Thin Layer Chromatography and the In silico studies are evident for the binding capacity of Anti-Oxidant Activity as 74% and Anti In inflammatory Activity as 72% which can a scope for future determination. Conclusion: The current study was notably focused on exploring new bioactive compounds that could be beneficial in the development of natural medicines targeting blood purification and its associated health benefits.