Reversed phase high-performance liquid chromatography for the evaluation of Luliconazole in bulk and pharmaceutical preparations

A new precise economical RP-HPLC analytical approach will serve as the basis for Luliconazole assay determination – both in bulk drug form and topical cream format. The separation took place on a HPLC device. The chromatographic separation was performed on a reverse-phase C-18 column (250 mm × 4.6 mm, 5 µ) using 0.1% OPA: acetonitrile (75:25) as the mobile phase at a flow rate of 1.10 mL/min. The wavelength of 298 nm was employed for detecting the effluents through the system. The physical measurement revealed that Luliconazole exists at 3.112 minutes, and the determined correlation coefficient value for Luliconazole measurement reached 0.996, while Luliconazole measurement in the formulation range from 98% to 102%. The study validated the Luliconazole (LOD) and limit of quantification (LOQ) values of 1.31 µg/mL and 3.98 µg/mL, respectively. Its simplicity, accuracy, rapid execution and ease of implementation revealed a successful application of method for the analysis of Luliconazole in bulk drug and topical cream formulations. Słowa kluczowe: cream, luliconazole, method development, RP-HPLC, validation