The Impact of Cytopathogenic and Non-cytopathogenic Biotypes of Bovine Viral Diarrhea Virus on Total Antioxidant Capacity of Bovine Oocytes In-vitro

Background: Bovine viral diarrhea virus (BVDV) is the most common pathogen in dairy cattle herds. Objectives: This study aims to examine the impact of cytopathic (CP) and non-cytopathic (NCP) BVDV biotypes on the total antioxidant capacity (TAC) of bovine oocytes in vitro. Methods: Oocytes were obtained from slaughtered bovine ovaries, washed, and matured in a maturation medium. Oocytes were divided into five groups, each consisting of at least 60. The control group was not exposed to BVDV biotypes. Oocytes were challenged with CP and NCP, BVDV at two concentrations of 104 and 105 tissue culture infectious doses (TCID)50/mL. To determine the antioxidant capacity of oocytes, a TAC assay was conducted after two hours of incubation. Graph Pad Prism software, version 8.4.3 was utilized to analyze the data. A one-way analysis of variance (ANOVA) was performed, and a post-hoc Tukey’s honestly significant difference (HSD) test was accompanied. Results: The results indicated that only the CP biotype of BVDV significantly decreased the TAC of infected oocytes compared to the control group, whereas the NCP biotype did not significantly alter the TAC of the infected groups. Conclusion: Our study showed that only CP BVDV in 104 and 105 TCID50/mL doses affected the infected oocytes and significantly decreased the oocyte’s TAC. At the same time, the NCP biotype did not significantly alter the infected oocyte’s TAC.