Temporal and thermal optimization of trypsin digestion for the cryptococcal proteome

Proteomics investigates diverse biological systems, providing insights into regulation, signaling, modifications, and interactions. Optimized sample preparation is critical to ensure robust and reproducible analyses balanced with time and cost. Herein, we evaluated trypsin digestion parameters for the human fungal pathogen, <i>Cryptococcus neoformans</i>, and determined conditions for optimal protein identification.