Amniotic fluid MSCs for scaffold-free cartilage repair: spheroid fusion and chondrogenic microtissue development
AF-MSCs differentiated into chondrocytes when stimulated with TGF-β3 in a 3D micromolded culture, forming uniform, viable spheroids with robust ECM production and mechanical properties. These spheroids fused into neocartilage microtissue, showing potential for regenerative medicine, especially osteoarthritis treatment and drug testing. Further research should optimize conditions and evaluate long-term biomechanical performance.
Authors
- Ângela Maria Moraes (ORCID: https://orcid.org/0000-0002-5813-332X)
- Ibsen Bellini Coimbra (ORCID: https://orcid.org/0000-0001-6429-4878)
- C.C. Zuliani (ORCID: https://orcid.org/0000-0002-5538-2726)
- Victor Marchiori de Souza (ORCID: https://orcid.org/0000-0002-7193-4017)
- Kléber Cursino de Andrade (ORCID: https://orcid.org/0000-0002-0824-3504)
- Jéssica Bruna da Cunha (ORCID: https://orcid.org/0000-0002-6170-3960)
Institutions
- Universidade Estadual de Campinas (UNICAMP) (BR)
Publication Details
- Journal
- Future Science OA
- Published
- 2025-06-11
- DOI
- https://doi.org/10.1080/20565623.2025.2476922
- Citations
- 2
- Primary Topic
- Osteoarthritis Treatment and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 3.34
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico