A plant Lysin Motif Receptor-Like Kinase plays an ancestral function in mycorrhiza
Arbuscular mycorrhiza (AM) with soilborne Glomeromycota fungi was pivotal in the conquest of land by plants almost half a billion years ago. In flowering plants, it is hypothesized that AM is initiated by the perception of AM fungi-derived chito- and lipochito-oligosaccharides (COs/LCOs) in the host via Lysin Motif Receptor-Like Kinases (LysM-RLKs). However, it remains uncertain whether plant perception of these molecules is a prerequisite for AM establishment and for its origin. Here, we made use of the reduced LysM-RLK complement present in the liverwort <i>Marchantia paleacea</i> to assess the conservation of the role played by this class of receptors during AM and in CO/LCO perception. Our reverse genetic approach demonstrates the critical function of a single LysM-RLK, MpaLYKa, in AM formation, thereby supporting an ancestral function for this receptor in symbiosis. Binding studies, cytosolic calcium variation recordings and genome-wide transcriptomics indicate that another LysM-RLK of <i>M. paleacea</i>, MpaLYR, is also required for triggering a response to COs and tested LCOs, despite being dispensable for AM formation. Collectively, our results demonstrate that the perception of symbionts by LysM-RLK is an ancestral feature in land plants, and suggest the existence of yet-uncharacterized AM fungi signals.
Authors
- Pierre‐Marc Delaux (ORCID: https://orcid.org/0000-0002-6211-157X)
- Benoît Lefebvre (ORCID: https://orcid.org/0000-0002-3810-605X)
- Sabine Grat
- Mélanie K. Rich (ORCID: https://orcid.org/0000-0001-6109-1290)
- Jean Keller (ORCID: https://orcid.org/0000-0002-5198-0331)
- Malick Mbengué (ORCID: https://orcid.org/0000-0002-7723-7757)
- Sébastien Fort (ORCID: https://orcid.org/0000-0002-6133-9900)
- David Landry (ORCID: https://orcid.org/0000-0002-6147-8580)
- Eve Teyssier
- Mathilde Ouradou
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- Institut National Polytechnique de Toulouse (FR)
- Centre de Recherches sur les Macromolécules Végétales (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Laboratoire de Recherche en Sciences Végétales (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Proceedings of the National Academy of Sciences
- Published
- 2025-06-11
- DOI
- https://doi.org/10.1073/pnas.2426063122
- Citations
- 9
- Primary Topic
- Legume Nitrogen Fixing Symbiosis
- Type
- article
- Field-Weighted Citation Impact
- 15.75
Funders
- Bill and Melinda Gates Foundation
- LABoratoires d’EXcellence ARCANE
- Laboratoire d'Excellence TULIP
- European Commission
- Agence Nationale de la Recherche
- Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
- Institut Carnot PolyNat
- Région Occitanie Pyrénées-Méditerranée
- Foreign, Commonwealth and Development Office
- HORIZON EUROPE European Research Council