Comparative evaluation of four new immunoassays and LC-MS/MS for the measurement of urinary free cortisol in Cushing's syndrome diagnosis
Four newly available direct immunoassays for measuring UFC show good analytical consistency compared to LC-MS/MS. The elimination of organic solvent extraction simplifies workflows while maintaining high diagnostic accuracy. Additionally, they exhibited similarly high diagnostic accuracy for CS identification. Future multi-center studies are needed to validate our findings and establish method-specific UFC cut-off values to enhance clinical utility.
Authors
- Yuemeng Li (ORCID: https://orcid.org/0000-0002-8796-1503)
- Xinqi Cheng (ORCID: https://orcid.org/0000-0001-8228-5078)
- Yumeng Gao (ORCID: https://orcid.org/0000-0001-6990-2203)
- Ran Gao (ORCID: https://orcid.org/0000-0003-0331-1958)
- Qi Zhang (ORCID: https://orcid.org/0000-0003-1121-8817)
- Kui Zhang (ORCID: https://orcid.org/0000-0002-8370-8412)
- Huijuan Zhu
- Yichen Ma
- Liangyu Xia (ORCID: https://orcid.org/0000-0002-9900-4479)
- Songlin Yu (ORCID: https://orcid.org/0000-0002-5284-9730)
- Fang Zhao
- Ling Qiu
- Yingying Hu (ORCID: https://orcid.org/0009-0002-5909-8252)
- Danni Mu (ORCID: https://orcid.org/0000-0003-0787-0538)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
Publication Details
- Journal
- Practical Laboratory Medicine
- Published
- 2025-06-11
- DOI
- https://doi.org/10.1016/j.plabm.2025.e00484
- Primary Topic
- Pituitary Gland Disorders and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00