The Growth-Inhibitory Effect of Glass Ionomer Liners Reinforced with Fluoride-Modified Nanotubes

The aim of this research was to compare the growth-inhibitory effect of halloysite-based nanotubes preloaded with sodium fluoride incorporated into two commercial glass ionomers (Vitrebond 3MTM and Ionobond VOCO) for indirect pulp capping. Methods: Sixty samples were prepared and were distributed into two control groups, two positive control groups and two experimental groups. A total of 10% of the total weight of ionomer powder required to prepare each sample was replaced with nanotubes that had been preloaded at 2000 parts per million (minimum inhibitory dose for Streptococcus mutans established in this study using the McFarland index). The growth-inhibitory effect was determined by placing the samples in Petri dishes inoculated with S. mutans for 24 h at 37 °C. Results: Regarding the control groups, only Vitrebond demonstrated a growth inhibition zone; both experimental groups showed an inhibitory effect, and statistical differences were observed when the experimental Ionobond group and control groups were compared. Conclusions: The ionomers reinforced with fluorine-modified nanotubes showed an adequate inhibitory effect on Streptococcus mutans.