Efficient Degradation of Consumer-Grade PLA by Commercial Savinase: Optimized Conditions and Molecular Dynamics Insights

Poly-(lactic acid) (PLA) is the most widely produced biopolymer globally, with 920,000 tons reaching the market in 2024. Although it is a biobased polymer and biodegradable, it currently poses a threat by generating a considerable waste stream in the environment unless its end-of-life options are further developed. Biorecycling of PLA is a promising solution and is critical for PLA to be a truly sustainable alternative to conventional plastics, further enabling the (bio)-plastics circular economy. This work investigates the repurposing of commercial Savinase 12T preparation, already produced on a large scale for the detergent industry, for the degradation of consumer-grade PLA. Savinase-degraded postconsumer PLA single-use cups at a rate of 166 mg·day^-1·mg enzyme^-1 for the lid part (crystallinity (<i>X</i> _c) 22.9%) and 40 mg·day^-1·mg enzyme^-1 for the body part (<i>X</i> _c 42.0%). The average degradation rate of PLA 3D printer filaments was determined to be 56 mg·day^-1·mg enzyme^-1 under optimized conditions of pH 8.5 and 42 °C. Lactic acid was identified as the main PLA degradation product of Savinase depolymerizing activity. Molecular modeling revealed Asn155 and Ser125 as key residues involved in PLA tetramer binding.