Intrinsic fluorescence in Moringa Oleifera seed extract

• Report of intrinsic fluorescence in Moringa oleifera seed extract, enabling noninvasive bioactive compound identification. • Amino acids (tyrosine, phenylalanine) and polyphenolic compounds contribute to the observed autofluorescence. • Autofluorescence-based detection offers a novel tool for quality assessment, pharmaceutical research, and functional food authentication. Autofluorescence, an intrinsic property of biological molecules that emit light upon excitation, is gaining significance as a noninvasive technique for studying plant-derived bioactive compounds. This phenomenon enables detection of biomolecules such as chlorophyll, lignin, flavonoids, and alkaloids without requiring external dyes or destructive extraction processes. Intrinsic fluorescence can also aid in detecting and identifying biomolecules that typically require extensive sample preparation (molecule labelling with external fluorophores), eliminating the need for external probes. The present study aims to report the intrinsic autofluorescence in the Moringa Oleifera plant, highlighting its potential to enhance noninvasive bioactive compound identification and quality assessment in plant-based products. Graphical representation of Autofluorescence of Moringa Oleifera seed extract as seen through confocal microscope and its emission spectra obtained.