Ameliorative Effects of Honey on Petrol Vapor-Induced Oxidative Stress in the Reproductive System of Female Wistar Rats

Background and Purpose: Oxidative stress and reproductive dysfunction have been linked to petroleum exposure. This study investigated the protective effects of honey on oxidative stress markers, reproductive hormones, and ovarian histology in female Wistar rats exposed to gasoline vapor. Methods: Fifty-six female rats were randomly divided into seven groups including control, 6- or 12-weeks gasoline-exposed, and 6- or 12-weeks gasoline-exposed alongside honey at 330 mg/kg/day or 1000 mg/kg/day doses. Results: Gasoline vapor exposure induced significant oxidative stress, evidenced by increased catalase and superoxide dismutase activities, and elevated malondialdehyde (MDA) levels. Hormonal analysis revealed significant alterations, particularly reduced luteinizing hormone (LH), estrogen, and progesterone, and increased follicle-stimulating hormone (FSH) in exposed rats. Histology showed follicular degeneration and reduced corpora lutea. Honey treatment significantly restored antioxidant enzyme activity, normalized hormone levels, and improved follicular development and ovarian morphology. Conclusion: These findings suggest that honey mitigates gasoline vapor-induced reproductive toxicity through its antioxidant and hormone-regulatory properties.