Effect of Clostridium butyricum and Bacillus coagulans on fecal and serum metabolic profiles in Helicobacter pylori-infected mice

This study aims to explore the potential mechanism of H. pylori eradication by Clostridium butyricum and Bacillus coagulans through metabolomics. A mouse model of H. pylori infection was established by gavage of H. pylori solution into mice. Mice of the probiotic treatment group were given probiotics suspension. Then, the mouse feces and serum of all mice were collected for metabolomics analysis. The eradication rate of probiotics was 62.5 %, and it significantly altered the metabolic profiles of feces and serum in H. pylori -infected mice. Probiotics reversed 25 fecal and 18 serum metabolites. These metabolites are involved in the metabolic pathways related to linoleic acid metabolism, primary bile acid biosynthesis, arachidonic acid metabolism and lipid metabolism. This study demonstrated that two specific probiotics exhibited a significant capacity to partially eradicate H. pylori . Furthermore, we have provided preliminary insights into the eradication efficacy and underlying mechanisms of two probiotics against H. pylori . • This study proposes a novel non-antibiotic therapeutic strategy for H. pylori . • Multi-omics profiling reveals the therapeutic mechanisms of probiotic treatment. • This discovery expands the clinical applications of probiotic-based interventions. • Utilizing a laboratory mice model of H. pylori improves the reliability of outcomes.