STR Typing of Autopsy Samples with Rapid DNA Extraction Reagent

STR typing of crime scene evidence is currently contributing to solving various cases. In a autopsy case, the identity of the body is confirmed by collecting blood and comparing the STR typing results of the deceased with those of the family. This study, the rapid DNA extraction reagent was used to purify DNA from autopsy samples, blood and costal cartilage, and the effect of the rapid DNA extraction reagent on STR type was examined by comparing it with standard DNA. As a result of the experiment, the average DNA concentration using the rapid extraction reagent was purified to 0.277 ng/㎕ for blood and 0.069 ng/㎕ for costal cartilage, which met the recommended DNA concentration of 0.050 ng/㎕ for the STR amplification kit. The DNA degradation index was confirmed to be a maximum of 1.703 for blood and 0.660 for costal cartilage. In the case of STR type detection, DNA types were detected in all blood and costal cartilage samples. In the balance comparison of the values of heterozygous loci, there was no significant difference in the analysis values compared to the standard DNA samples, with 3 loci in blood, 6 loci in costal cartilage, and 6 loci in 007.