Liquid Y2H‐Seq, a Rapid and Data‐Rich Alternative to Conventional Yeast Two‐Hybrid Screening
Highly laborious plating of cultures onto solid media is inevitable in conventional yeast two-hybrid systems. We have successfully developed Liquid Y2H-Seq, a method that replaces solid media with liquid media for culturing and displaces visual inspection of colonies with sequencing. Soybean (Glycine max) is a typical photoperiod-sensitive crop, meaning that a specific duration of light regime has a huge impact on soybean flowering and production. We obtained a lot of putative interactions for soybean flowering genes. Finally, these reports introduce a labor-reducing and time-saving method for identifying protein-protein interactions, based on simple modifications to a ubiquitous protocol in life science research.
Authors
- Tianfu Han (ORCID: https://orcid.org/0000-0002-2362-2414)
- Bingjun Jiang (ORCID: https://orcid.org/0000-0002-8172-4646)
- Yanping Qi (ORCID: https://orcid.org/0000-0001-9352-7140)
- Cunxiang Wu
- Shi Sun (ORCID: https://orcid.org/0000-0003-1422-5334)
- Chunlei Zhang (ORCID: https://orcid.org/0000-0002-0939-2149)
- Lixin Zhang (ORCID: https://orcid.org/0000-0003-0268-6774)
- Xinyi Xu (ORCID: https://orcid.org/0000-0002-1361-2829)
- Luping Liu (ORCID: https://orcid.org/0000-0001-8644-7994)
- Tingting Wu (ORCID: https://orcid.org/0000-0003-3464-1511)
- Shan Yuan (ORCID: https://orcid.org/0000-0002-2936-6589)
Institutions
- Chinese Academy of Agricultural Sciences (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Institute of Crop Sciences (CN)
Publication Details
- Journal
- PROTEOMICS
- Published
- 2025-06-08
- DOI
- https://doi.org/10.1002/pmic.13970
- Primary Topic
- Bioinformatics and Genomic Networks
- Type
- article
- Field-Weighted Citation Impact
- 0.00