THE EFFECTIVENESS OF DRY ETHANOL EXTRACTS OF AGRIMONIA PILOSA LEDEB. AND SOME OTHER PLANTS OF THE ROSOIDEAE SUBFAMILY OF THE ROSACEAE FAMILY FOR INACTIVATION OF SARS-COV-2 AND HERPES SIMPLEX VIRUS 2 TYPE

Such representatives of the Rosoideae subfamily of the Rosaceae family Agrimonia pilosa Ledeb., Alchemilla vulgaris L., Sanguisorba officinalis L., Sanguisorba alpina Bunge and Geum rivale L. in nature Russian Federation are widespread. The antiviral activity and phenolic composition of dry ethanol extracts of individual parts (leaves, flowers and stems) these plants have been studied. The extracts were dissolved in DMSO and in the reaction of direct inactivation (neutralization) of virus in vitro their activity against two pathogens the SARS-CoV-2 and HSV-2 in the range of 50% effective concentrations of 1.09±0.24 – 50.78±11.2 μg/ml was revealed. It has been shown that the extracts exhibit low cytotoxicity which affects the values of the selectivity indices. Flavonoid cynaroside (0.79%) have been identified in the leaves of A. pilosa. In extracts of other Rosoideae spp. chemical compounds such as chlorogenic acid, rutin, quercetin, isoquercitrin, quercitrin hydrate, epicatechin, vicenin-2 and several unidentified flavonoids have been identified. Based on the data obtained, it can be assumed that the activity of the studied ethanol extracts of Rosoideae spp. during direct inactivation (neutralization), SARS-CoV-2 and HSV-2 are associated with polyphenolic compounds. The results obtained can become the basis for the search for individual plant-based biologically active substances that inhibit the "entry" of these viruses into a sensitive cell, as well as for the development of inexpensive drugs for the prevention and/or treatment of COVID-19 and to reduce the recurrence of chronic genital herpes.