Experimental Platform for Investigation of Low-Frequency Magnetic Field Effects on Cells

Abstract This study presents a novel experimental platform designed to systematically investigate the effects of low-frequency (LF) magnetic fields (MFs) on biological systems. By overcoming key methodological challenges, including variability in environmental conditions and poor reproducibility, this platform sets a new standard for experimental reliability. It integrates precise temperature regulation, high MF homogeneity, and a modular structure, that ensures adaptability to diverse experimental setups. The platform was validated using Saccharomyces cerevisiae CCY 21-4-99 as a model organism, cultivated under controlled conditions with and without MF exposure. The minimal growth variations observed between chambers confirm the ability of the platform to maintain reproducible conditions and support statistically robust experimental designs. The platform can be applied to diverse biological systems and can be technically adapted to different experimental requirements, including different electromagnetic field sources. It provides a highly controlled environment for investigating the cellular and subcellular effects of LF MF, creating a solid foundation for future research into its biological mechanisms and potential applications.