Effects of Cholecalciferol on the White Blood Cell Parameters following Traumatic Brain Injury Using Rat Model

Traumatic brain injury (TBI) is a significant cause of illness, disability, and mortality across all age groups. Every year, almost 50 million individuals worldwide suffer from TBI. By 2005, around 3.17 million TBI survivors were dealing with long-term repercussions, such as neurological, psychological, and irreversible disability. Vitamin D may modulate the immune system, according to research, since its metabolites and receptors (VDR) are generated and expressed in white blood cells. This research is to evaluate the impact of cholecalciferol on white blood cell parameters after TBI. A total of 42 adult Wistar rats weighing between 180 and 230g were randomly split into four groups (A, B, C, and D) of 12 rats each, with additional subgroups of six rats. Group A functioned as the control group, and no brain damage occurred. Group B was subjected to brain damage with a 100g weight; sub-group B1 got no therapy, whilst sub-group B2 received cholecalciferol. Group C was given a 200g weight; sub-group C1 went untreated, whereas sub-group C2 got therapy. Group D was given a 300g weight; sub-group D1 was left untreated, while sub-group D2 received cholecalciferol. The brain damage were caused by the Marmarou’s weight-drop equipment. Blood samples were taken from all rats one week before TBI induction to establish baseline values, as well as after 6, 24, and 72 hours after the shock. These samples were examined at a haematology lab to determine differential white blood cell counts. White blood cell counts and differentials did not alter significantly after cholecalciferol treatment. Following a TBI, cholecalciferol has no substantial impact on white blood cells or their parameters. Keywords: Traumatic brain injury, Cholecalciferol, White Blood Cell, metabolites and receptors