High-throughput robotic isolation of human iPS cell clones reveals frequent homozygous induction of identical genetic manipulations by CRISPR-Cas9
This study revealed a previously unknown property of genome editing in human iPS cells that identical sequence manipulations tend to be induced in both copies of the target sequence in individual cells. Our new cloning method and findings will facilitate the application of genome editing to human iPS cells.
Authors
- Gakuro Harada
- Gou Takahashi (ORCID: https://orcid.org/0000-0003-2277-7657)
- Yuichiro Miyaoka (ORCID: https://orcid.org/0000-0002-8874-4738)
- Minato Maeda
- Kayoko Shinozaki
- Saburo ITO
Institutions
- Tokyo Medical and Dental University (JP)
- Tokyo University of Agriculture (JP)
- Yamaha (Japan) (JP)
- Ochanomizu University (JP)
- Tokyo Metropolitan Institute of Medical Science (JP)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-06-07
- DOI
- https://doi.org/10.1186/s13287-025-04414-2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Takeda Science Foundation
- Sumitomo Foundation
- Japan Agency for Medical Research and Development
- Yamaha Motor Company
- Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care
- Japan Society for the Promotion of Science