CRISPR-Cas9: A Tool Used in Plant Breeding

CRISPR-Cas9, a revolutionary tool for genome editing, has significantly impacted plant breeding by allowing precise genetic modifications with exceptional efficiency and accuracy. This review explores the applications, mechanisms, and implications of CRISPR-Cas9 technology in plant breeding. It outlines the molecular principles of the CRISPR-Cas9 system, focusing on its ability to target specific genes and introduce traits like disease resistance, enhanced yield, and better resilience to environmental stresses. The review highlights the advantages of CRISPR-Cas9 over traditional breeding methods, such as its speed, cost-effectiveness, and precision. Additionally, it addresses the challenges and regulatory issues surrounding its use in agriculture, including off-target effects, ethical concerns, and regulatory frameworks. The review concludes by considering the future potential of CRISPR-Cas9 in fostering sustainable agricultural practices and its role in ensuring global food security in the face of climate change and population growth.

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Publication Details

Journal
Journal of Experimental Agriculture International
Published
2025-06-07
DOI
https://doi.org/10.9734/jeai/2025/v47i63496
Primary Topic
CRISPR and Genetic Engineering
Type
article
Field-Weighted Citation Impact
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article

CRISPR-Cas9: A Tool Used in Plant Breeding

Durga Prasad, JS Thakur, Nishant Gharia, M Sayooj
Journal of Experimental Agriculture International
CRISPR and Genetic Engineering
article

CRISPR-Cas9: A Tool Used in Plant Breeding

Durga Prasad, JS Thakur, Nishant Gharia, M Sayooj
article en

Abstract

CRISPR-Cas9, a revolutionary tool for genome editing, has significantly impacted plant breeding by allowing precise genetic modifications with exceptional efficiency and accuracy. This review explores the applications, mechanisms, and implications of CRISPR-Cas9 technology in plant breeding. It outlines the molecular principles of the CRISPR-Cas9 system, focusing on its ability to target specific genes and introduce traits like disease resistance, enhanced yield, and better resilience to environmental stresses. The review highlights the advantages of CRISPR-Cas9 over traditional breeding methods, such as its speed, cost-effectiveness, and precision. Additionally, it addresses the challenges and regulatory issues surrounding its use in agriculture, including off-target effects, ethical concerns, and regulatory frameworks. The review concludes by considering the future potential of CRISPR-Cas9 in fostering sustainable agricultural practices and its role in ensuring global food security in the face of climate change and population growth.

Journal of Experimental Agriculture InternationalVol. 47(6)
Openalex Percentile: Top 88%
CRISPR and Genetic Engineering
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CRISPR-Cas9: A Tool Used in Plant Breeding — Durga Prasad, JS Thakur, et al. · Journal of Experimental Agriculture International (2025) | TGRS Research Map | TGRS