3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline
We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.
Authors
- Denis Vivien (ORCID: https://orcid.org/0000-0002-7636-2185)
- Sylvain Bodard (ORCID: https://orcid.org/0000-0001-5381-3361)
- Cyrille Orset (ORCID: https://orcid.org/0000-0002-9139-3430)
- Louise Denis (ORCID: https://orcid.org/0000-0001-6395-7377)
- Arthur Chavignon (ORCID: https://orcid.org/0000-0001-7883-7482)
- Olivier Couture (ORCID: https://orcid.org/0000-0002-8683-1424)
- Jacques Battaglia
- Georges Chabouh (ORCID: https://orcid.org/0000-0003-0760-909X)
- Baptiste Heiles (ORCID: https://orcid.org/0000-0001-9254-1741)
- Jean-Baptiste Deloges (ORCID: https://orcid.org/0000-0001-5753-1994)
- Myriam Abioui-Mourgues (ORCID: https://orcid.org/0000-0001-7533-4070)
- Basile Pradier
Institutions
- California Institute of Technology (US)
- Hôpital Necker-Enfants Malades (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Sorbonne Université (FR)
- Sorbonne Paris Cité (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Normandie Université (FR)
- Centre Hospitalier Universitaire de Caen Normandie (FR)
- Laboratoire d’Imagerie Biomédicale (FR)
- Cyceron (FR)
- Université Paris 1 Panthéon-Sorbonne (FR)
- Université de Caen Normandie (FR)
Publication Details
- Journal
- Communications Engineering
- Published
- 2025-06-06
- DOI
- https://doi.org/10.1038/s44172-025-00415-4
- Citations
- 3
- Primary Topic
- Ultrasound Imaging and Elastography
- Type
- article
- Field-Weighted Citation Impact
- 4.08