Fecal Microbiota Transplantation from Mice Receiving Magnetic Mitohormesis Treatment Reverses High-Fat Diet-Induced Metabolic and Osteogenic Dysfunction

This study compared the metabolic consequences of fecal microbiota transplantation (FMT) from donor mice that had been either administered pulsed electromagnetic field (PEMF) therapy or exercised to recipient mice fed a high-fat diet (HFD). Eight weeks of PEMF treatment (10 min/week) enhanced PGC-1α-associated mitochondrial and metabolic gene expression in white and brown adipose to a greater degree than eight weeks of exercise (30-40 min/week). FMT from PEMF-treated donor mice recapitulated these adipogenic adaptations in HFD-fed recipient mice more faithfully than FMT from exercised donors. Direct PEMF treatment altered hepatic phospholipid composition, reducing long-chain ceramides (C16:0) and increasing very long-chain ceramides (C24:0), which could be transferred to PEMF-FMT recipient mice. FMT from PEMF-treated mice was also more effective at recovering glucose tolerance than FMT from exercised mice. PEMF treatment also enhanced bone density in both donor and HFD recipient mice. The gut Firmicutes/Bacteroidetes (F/B) ratio was lowest in both the directly PEMF-exposed and PEMF-FMT recipient mouse groups, consistent with a leaner phenotype. PEMF treatment, either directly applied or via FMT, enhanced adipose thermogenesis, ceramide levels, bone density, hepatic lipids, F/B ratio, and inflammatory blood biomarkers more than exercise. PEMF therapy may represent a non-invasive and non-strenuous method to ameliorate metabolic disorders.

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Publication Details

Journal
International Journal of Molecular Sciences
Published
2025-06-06
DOI
https://doi.org/10.3390/ijms26125450
Citations
1
Primary Topic
Diet and metabolism studies
Type
article
Field-Weighted Citation Impact
1.16

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article

Fecal Microbiota Transplantation from Mice Receiving Magnetic Mitohormesis Treatment Reverses High-Fat Diet-Induced Metabolic and Osteogenic Dysfunction

Tuan Zea Tan, Shabbir Moochhala, Abdul Jalil Rufaihah, Yee Kit Tai et al.
1 citations
International Journal of Molecular Sciences
Diet and metabolism studies
1.16
article

Fecal Microbiota Transplantation from Mice Receiving Magnetic Mitohormesis Treatment Reverses High-Fat Diet-Induced Metabolic and Osteogenic Dysfunction

Tuan Zea Tan, Shabbir Moochhala, Abdul Jalil Rufaihah, Yee Kit Tai, Wei Wei Thwe Khine, Lye Siang Lee, Jan Nikolas Iversen, Kristy Purnamawati, Way Cherng Chen, Alan Prem Kumar, Yuan Kun Lee, Bharati Kadamb Patel, Tsze Yin Tan, Jianhong Ching, Marek Kukumberg, Craig Jun Kit Wong, Alfredo Franco‐Obregón, Xizhe Bai, Kee Voon Chua, Kwan Yu Wu
article en
1 citations

Abstract

This study compared the metabolic consequences of fecal microbiota transplantation (FMT) from donor mice that had been either administered pulsed electromagnetic field (PEMF) therapy or exercised to recipient mice fed a high-fat diet (HFD). Eight weeks of PEMF treatment (10 min/week) enhanced PGC-1α-associated mitochondrial and metabolic gene expression in white and brown adipose to a greater degree than eight weeks of exercise (30-40 min/week). FMT from PEMF-treated donor mice recapitulated these adipogenic adaptations in HFD-fed recipient mice more faithfully than FMT from exercised donors. Direct PEMF treatment altered hepatic phospholipid composition, reducing long-chain ceramides (C16:0) and increasing very long-chain ceramides (C24:0), which could be transferred to PEMF-FMT recipient mice. FMT from PEMF-treated mice was also more effective at recovering glucose tolerance than FMT from exercised mice. PEMF treatment also enhanced bone density in both donor and HFD recipient mice. The gut Firmicutes/Bacteroidetes (F/B) ratio was lowest in both the directly PEMF-exposed and PEMF-FMT recipient mouse groups, consistent with a leaner phenotype. PEMF treatment, either directly applied or via FMT, enhanced adipose thermogenesis, ceramide levels, bone density, hepatic lipids, F/B ratio, and inflammatory blood biomarkers more than exercise. PEMF therapy may represent a non-invasive and non-strenuous method to ameliorate metabolic disorders.

International Journal of Molecular SciencesVol. 26(12)
National University of Singapore (SG), Temasek Polytechnic (SG), University of Zurich (CH), National University Cancer Institute, Singapore (SG), KK Women's and Children's Hospital (SG), Duke-NUS Medical School (SG), National University Health System (SG)
National University of Singapore, Ministry of Education - Singapore, Ministry of Education, India, National University Health System, Institute for Health Innovation and Technology, National University of Singapore
Openalex Percentile: Top 21%
Diet and metabolism studies
1.16
DOIOpen Access PDFOpenAlex
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