Modular and signal-responsive transcriptional regulation using CRISPRi-aided genetic switches in Escherichia coli
The CRISPRi-aided genetic switch provides a powerful and flexible toolkit for synthetic biology, addressing the limitations of existing systems. By enabling precise and tunable transcriptional regulation, it offers robust solutions for a wide array of biotechnological applications, including pathway engineering and synthetic gene networks.
Authors
- Seung‐Goo Lee (ORCID: https://orcid.org/0000-0003-4539-9812)
- Seong Keun Kim (ORCID: https://orcid.org/0000-0001-8712-7167)
- Seung‐Gyun Woo
- Jun Hong Park (ORCID: https://orcid.org/0000-0001-8905-4125)
- Dae‐Hee Lee (ORCID: https://orcid.org/0000-0002-0423-9057)
Institutions
- Korea Research Institute of Bioscience and Biotechnology (KR)
- The University of Texas at Austin (US)
- Korea University of Science and Technology (KR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-06-06
- DOI
- https://doi.org/10.1186/s13036-025-00526-8
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Research Foundation
- Korea Research Institute of Bioscience and Biotechnology
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea