Design and Implementation of a Low-Cost Controlled-Environment Growth Chamber for Vegetative Propagation of Mother Plants

This Technical Note presents the design and implementation of a low-cost modular growth chamber developed to keep mother plants under controlled environmental conditions for vegetative propagation. The system was conceived as an accessible alternative to expensive commercial equipment, offering reproducibility and adaptability for small-scale and research-based cultivation. The proposed chamber integrates thermal insulation, LED lighting, forced ventilation through the implementation of extractors, a recirculating irrigation system with double filtration, and a sensor-based environmental monitoring platform operated via an Arduino UNO microcontroller. The design features a removable tray that serves as a support for the mother plant, an observation window covered by a movable dark acrylic that prevents the passage of external light, and a vertical structure that facilitates optimal space utilization and ergonomic access. Functionality was conducted using a Stevia rebaudiana Bertoni mother plant maintained for 30 days under monitored conditions. Environmental parameters—temperature, relative humidity, and illuminance—were recorded continuously. The plant showed vegetative development through new shoot emergence and the growth in height of the plant, and despite a loss in foliage expansion, it confirmed the chamber’s capacity to support sustained growth. Although no statistical replication or control group was included in this preliminary evaluation, the system demonstrates technical feasibility and practical utility. This chamber provides a replicable platform for future experimentation and propagation studies. Complete technical specifications, schematics, and component lists are provided to enable replication and further development by other researchers. The growth chamber design aligns with the goals of open-source agricultural innovation and supports knowledge transfer in controlled-environment plant propagation technologies.