Carbapenem resistance gene blaNDM-1 in Escherichia coli, Serratia marcescens and Klebsiella michiganensis strains from a pediatric patient
Carbapenem resistance has a widespread globally. In this study, three bacterial strains including Escherichia coli (SXY-EC), Serratia marcescens (SXY-SM), and Klebsiella michiganensis (SXY-KM) were isolated from a pediatric patient in China. Whole-genome sequencing and resistome analysis revealed the appearance of bla<sub>NDM-1</sub> β-lactamase gene in all these three strains, and conjugation tests confirmed its transferability. Additionally, SXY-SM and SXY-KM harbored two to three other resistance genes, while SXY-EC carried eleven. Notably, the multidrug resistance region containing bla<sub>NDM-1</sub> in plasmid pSXY-EC_NDM1 from SXY-EC was flanked by mobile elements, potentially enabling intermolecular replicative transposition mediated by distinct insertion sequences.
Authors
- Lili Huang (ORCID: https://orcid.org/0000-0001-8811-0922)
- Yunbing Li
- Mi Zhou (ORCID: https://orcid.org/0000-0001-9056-9468)
- Ning Dong (ORCID: https://orcid.org/0000-0001-6515-4201)
- Yong Liu (ORCID: https://orcid.org/0000-0003-1769-9789)
- Yuanyuan Li (ORCID: https://orcid.org/0000-0002-1497-6376)
- Xiaofan Li (ORCID: https://orcid.org/0000-0003-4379-3277)
- Chen Xu (ORCID: https://orcid.org/0000-0001-6980-0581)
- Xiangkun Zeng
Institutions
- Jiangsu University (CN)
- Soochow University (CN)
- Ruihua Affiliated Hospital of Soochow University (CN)
- Second Affiliated Hospital of Zhejiang University (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Infection and Public Health
- Published
- 2025-06-06
- DOI
- https://doi.org/10.1016/j.jiph.2025.102869
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jiangsu University
- Zhejiang University