A Versatile Sample Chamber for BioSAXS: Integration and Applications on the P12 Beamline

Abstract We present the integration of a sample chamber for advanced BioSAXS measurement on the P12 beamline of EMBL Hamburg. The sample chamber is designed to be installed in the beamline P12 at PETRA and BM29 at ESRF with minimal effort to swap the standard sample environment, allowing for a fast and efficient beamline configuration change. The new sample chamber is equipped with an on-axis microscope, including front and back illumination, for sample visualization; an XYZ piezo stage for fine alignment of sample into the beam; an optical port for in situ spectroscopy; and fluidics feedthroughs. This sample environment has been fully integrated on the P12 beamline and employed in users project for scanning SAXS and in vacuum microfluidics applications.

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Publication Details

Journal
Journal of Physics Conference Series
Published
2025-05-01
DOI
https://doi.org/10.1088/1742-6596/3010/1/012145
Primary Topic
Microfluidic and Capillary Electrophoresis Applications
Type
article
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article

A Versatile Sample Chamber for BioSAXS: Integration and Applications on the P12 Beamline

Florian D. C. Wieland, Gergely Papp, Martha Brennich, Mark D. Tully et al.
Journal of Physics Conference Series
Microfluidic and Capillary Electrophoresis Applications
article

A Versatile Sample Chamber for BioSAXS: Integration and Applications on the P12 Beamline

Florian D. C. Wieland, Gergely Papp, Martha Brennich, Mark D. Tully, Aleksi Sutinen, Serena DeBeer, Olivia McCubbin Stepanic, Dmytro Soloviov, Clément Blanchet, Petra Pernot, Raphaël Cohen-Aberdam, Emil Galikeev
article en

Abstract

Abstract We present the integration of a sample chamber for advanced BioSAXS measurement on the P12 beamline of EMBL Hamburg. The sample chamber is designed to be installed in the beamline P12 at PETRA and BM29 at ESRF with minimal effort to swap the standard sample environment, allowing for a fast and efficient beamline configuration change. The new sample chamber is equipped with an on-axis microscope, including front and back illumination, for sample visualization; an XYZ piezo stage for fine alignment of sample into the beam; an optical port for in situ spectroscopy; and fluidics feedthroughs. This sample environment has been fully integrated on the P12 beamline and employed in users project for scanning SAXS and in vacuum microfluidics applications.

Journal of Physics Conference SeriesVol. 3010(1)
European Synchrotron Radiation Facility (FR), Max Planck Institute for Chemical Energy Conversion (DE), European Molecular Biology Laboratory (FR), European Molecular Biology Laboratory (DE), Helmholtz-Zentrum Hereon (DE)
Openalex Percentile: Top 88%
Microfluidic and Capillary Electrophoresis Applications
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A Versatile Sample Chamber for BioSAXS: Integration and Applications on the P12 Beamline — Florian D. C. Wieland, Gergely Papp, et al. · Journal of Physics Conference Series (2025) | TGRS Research Map | TGRS