Antibacterial Activity of Methanol Extract on Cashew Nut Shell (<i>Anacardium occidentale Linn</i>.) As A Poultry Feed Additive Against <i>Escherichia coli</i> and <i>Salmonella sp</i> that Isolated From Poultry Excreta in Kendari South East Sulawesi

Abstract Providing feed additives that are combined with feed additives is one way to boost chicken farm productivity. Probiotics, prebiotics, and acidifiers are examples of feed additives that can be used as antibiotics because of their capacity to control conditions in the digestive tract. Cashew Nut Shell (CNSL) contain active compounds that can used to inhibit the growth and extinguish Escherichia coli and Salmonella sp. The purpose of this study was to determine the effectiveness of each of the bioactive substances produced from a methanol extract of cashew nut shell to have a greater antibacterial inhibition. The research design consisted of 6 treatments and 4 replications with treatment T1 (positive control: ciprofloxacin), T2(negative control DMSO 5%), T3 (12,5% CNSL), T4 (25% CNSL), T5 (50% CNSL), T6 (100% CNSL). The study used RAL (Completely Randomized Design) and further tests were carried out with the duncan test at 5% level to determine the difference between each replication, variabel observed was zone of antimicrobial inhibition. The result indicated significant difference (P&lt;0,01) that the highest inhibitory ability was found in highest concentration of CNSL Methanol extract, compared to positive control CNSL which has lower inhibition effect. As conclusion CNSL methanol extract antibacterial inhibition against Escherichia coli and Salmonella sp