MALDI-TOF-MS unveils the distribution of oligosaccharides produced by hydrolysis of lichen polysaccharides through acidic and oxidative methods – a comparative study
MALDI-TOF MS methods coupled with offline chromatographic data were used to compare the distribution of oligosaccharides generated from acidic or oxidative degradation (Fitdog) of high molecular weight polysaccharides (> 10 kDa) obtained from two lichens Lasallia pustulata and Cetraria islandica . MALDI allowed to quickly compare the kinetics of degradation on both models (starting from non-purified polysaccharides) and to evaluate the dispersity of the resulting oligosaccharides. MALDI-MS confirmed on one hand that TFA hydrolysis gave neutral oligosaccharides easy to correlate with the chemical formula. On the other hand, more structural diversity was evidenced using the Fitdog protocol. Deep analysis of the MALDI data highlighted the formation of by-products corresponding to modified oligosaccharides (e.g., intracyclic cleavages). • Hydrolysis of lichen polysaccharides by TFA and Fitdog methods • Two different depolymerization profiles depending of the degradation method according to both HPLC-ELSD and MALDI-TOF-MS • Clean depolymerization profile in acidic media • Important oxidation by-products with Fitdog methodology • By-products characterized as oxidative opening degradation of monosaccharides building-blocks
Authors
- Laurent Legentil (ORCID: https://orcid.org/0000-0003-1402-150X)
- Françoise Lohézic‐Le Dévéhat (ORCID: https://orcid.org/0000-0003-4307-4942)
- David Ropartz (ORCID: https://orcid.org/0000-0003-4767-6940)
- Simon Ollivier (ORCID: https://orcid.org/0000-0002-7671-1736)
- Oznur Yeni (ORCID: https://orcid.org/0000-0001-6171-3096)
- Vincent Ferrières (ORCID: https://orcid.org/0000-0002-2780-7774)
- Mathieu Fanuel (ORCID: https://orcid.org/0000-0001-8384-8266)
- Isabelle Compagnon (ORCID: https://orcid.org/0000-0003-2994-3961)
- Joël Boustié (ORCID: https://orcid.org/0000-0002-3936-3859)
- Solenn Ferron (ORCID: https://orcid.org/0000-0002-3509-9982)
- Hélène Rogniaux
- Camille Guitteny
- Mathis Ralaivao
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Institut des Sciences Chimiques de Rennes (FR)
- Biopolymères Interactions Assemblages (FR)
- École Nationale Supérieure de Chimie de Rennes (FR)
- Institut Lumière Matière (FR)
- Université de Rennes (FR)
Publication Details
- Journal
- International Journal of Mass Spectrometry
- Published
- 2025-06-06
- DOI
- https://doi.org/10.1016/j.ijms.2025.117473
- Citations
- 2
- Primary Topic
- Lichen and fungal ecology
- Type
- article
- Field-Weighted Citation Impact
- 6.05
Funders
- Agence Nationale de la Recherche