Real-time PCR-HRM assay for precise identification of <i>Entamoeba</i> species in diarrheal samples: clinical validation in tropical health settings
This study is the first to standardize qPCR-HRM for the detection and differentiation of Entamoeba species from India. The qPCR-HRM assay offers a sensitive, specific and cost-effective diagnostic tool, contributing to improved patient management.
Authors
- Puja Garg
- Sumeeta Khurana (ORCID: https://orcid.org/0000-0002-6004-6292)
- Priya Datta (ORCID: https://orcid.org/0000-0001-5608-8919)
- Rakesh Sehgal (ORCID: https://orcid.org/0000-0003-2910-4973)
- Pankaj Malhotra
- Sadhna Lal Bhasin
- Divya Rattan
- Surinder Rana
Institutions
- Aarupadai Veedu Medical College & Hospital (IN)
- Post Graduate Institute of Medical Education and Research (IN)
Publication Details
- Journal
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Published
- 2025-05-21
- DOI
- https://doi.org/10.1093/trstmh/traf059
- Primary Topic
- Amoebic Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00