Qualitative chemical analysis of cinnamon flavored e-cigarette vapor to identify compounds of interest that may affect cardiovascular function

Previous work on the impact of cinnamon flavored electronic cigarette vapor on heart function during early development in zebrafish indicate chemical compounds in the vapor, aside from nicotine, can significantly affect heart function. To determine which compounds were present in the vapor used in our prior study, non-targeted, qualitative gas chromatography/mass spectrometry was performed. Nicotine was found to be present in the nicotine-containing vapor, and it was confirmed to be absent in the nicotine-free vapor. Other chemical compounds that may have affected cardiovascular function in zebrafish were also identified in the analyzed vapors including cinnamaldehyde and eugenol among others.