ISOLATION, CHARACTERIZATION, FERMENTATION STUDIES OF THERMOTOLERANT AMYLOLYTIC YEAST

Amylolytic and Thermotolerant Yeast was isolated from bakery samples and soil samples from cooking area. The isolate was confirmed as Candia tropicalis from colony morphology, microscopic observations and biochemical tests. Yeast is growing and the amylase of isolate is also active at 42oC. The isolate C. tropicalis was resistant to azoles and Echinocandin drugs and susceptible to Amphotericin B. Soluble starch and Rice starch was used as substrate for ethanol fermentation. The rice starch was obtained from boiled rice and alkaline treatment. The substrates were hydrolysed by commercial amylase and Amylolytic C. tropicalis isolate. Commercial enzyme yielded 71 ±0.98 % and 70 ± 1.22 % hydrolysis for Soluble starch and Rice starch. Hydrolysis of Soluble starch and Rice starch by Simultaneous Saccharification and Fermentation studies yielded 87.1±0.2% and 85.2±0.9% of substrate hydrolysis. The isolate is reported to have 87.29±0.9% and 86±0.9%. of ethanol fermentation efficiency for soluble starch and rice starch at 10 grams level. Separate saccharification and fermentation yielded ethanol fermentation efficiency of 89.3±1.1% and 88.7±1.3% for soluble starch and rice starch with hydrolysis of 87.1±0.2% and 85.2±0.9%. The study claims to perform Simultaneous Saccharification and Fermentation studies using isolated amylolytic and thermotolerant C.tropicalis for efficient production of Bioethanol. This can be an efficient bioprocessing strain as ethanol production is carried out at higher temperatures proving mesophilic enzyme ineffective.