<i>Doublesex</i> gene in <i>Bombyx mori</i>: <scp>CRISPR</scp>‐mediated female‐to‐male sex reversal through exon skipping
This study demonstrates that precision editing of dsx splice sites can enforce unidirectional sex conversion in insects, disrupting female reproductive capacity without affecting males. Overall, these findings provide valuable insights into our understanding of gonadal development and insect population management. © 2025 Society of Chemical Industry.
Authors
- Yajie Yuan
- Lu Zheng (ORCID: https://orcid.org/0000-0002-2414-1671)
- Éric Westhof (ORCID: https://orcid.org/0000-0002-6172-5422)
- Zhangping Yang (ORCID: https://orcid.org/0000-0002-5479-8447)
- Xiaoling Tong (ORCID: https://orcid.org/0000-0002-2649-899X)
- Minjin Han (ORCID: https://orcid.org/0000-0003-1784-8676)
- Zhan‐Feng Ye
- Hai Hu (ORCID: https://orcid.org/0000-0002-5348-5641)
- Fangyin Dai (ORCID: https://orcid.org/0000-0002-0215-2177)
- Lulu Liu (ORCID: https://orcid.org/0000-0002-2563-3379)
- Yahui Zhang (ORCID: https://orcid.org/0000-0003-3281-9830)
- Zhengwen Yan (ORCID: https://orcid.org/0000-0002-3598-3321)
- Tingting Zhou (ORCID: https://orcid.org/0000-0002-0970-1204)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Southwest University (CN)
- Chengde Medical University (CN)
- Guizhou Academy of Agricultural Sciences (CN)
- Chengde Medical University (CN)
- Architecture et Réactivité de l'arN (FR)
- Ministry of Agriculture and Rural Affairs (CN)
- Institut de Biologie Moléculaire et Cellulaire (FR)
- Université de Strasbourg (FR)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-06-02
- DOI
- https://doi.org/10.1002/ps.8947
- Citations
- 2
- Primary Topic
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Type
- article
- Field-Weighted Citation Impact
- 3.63
Funders
- Natural Science Foundation of Chongqing
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities