Maternal loss of mouse Nlrp2 alters the transcriptome and DNA methylome in GV oocytes and impairs zygotic genome activation in embryos
This study demonstrates that NLRP2 is essential for shaping the transcriptome of GV oocytes and preimplantation embryos. Maternal loss of Nlrp2 negatively impacts ZGA. Our findings that the DNA methylome of Het and Nlrp2-null oocytes was subtly changed, and that gene-body DNA methylation differences did not correlate with gene expression differences, suggest that posttranscriptional changes in transcript stability, rather than altered transcription itself, are primarily responsible for the changed transcriptome of Nlrp2-null oocytes.
Authors
- Imen Chakchouk (ORCID: https://orcid.org/0000-0001-7748-9260)
- Simon Andrews (ORCID: https://orcid.org/0000-0002-5006-3507)
- Alvin K. To
- Ying‐Wooi Wan (ORCID: https://orcid.org/0000-0002-4354-7482)
- Momal Sharif (ORCID: https://orcid.org/0000-0001-8876-9946)
- Zahra Anvar (ORCID: https://orcid.org/0000-0002-5990-0068)
- Michael D. Jochum (ORCID: https://orcid.org/0000-0002-2398-356X)
- Hannah Demond (ORCID: https://orcid.org/0000-0003-4706-4792)
- Daniel C. Kraushaar
- Ignatia B. Van den Veyver (ORCID: https://orcid.org/0000-0002-0651-5924)
- Gavin Kelsey (ORCID: https://orcid.org/0000-0002-9762-5634)
- Mino-Kenudson Mari
Institutions
- Universidad de La Frontera (CL)
- Babraham Institute (GB)
- Baylor College of Medicine (US)
- University of Cambridge (GB)
- Wellcome/MRC Institute of Metabolic Science (GB)
- Neurological Research Institute (US)
- Instituto de Neurociencia Biomédica (CL)
- Texas Children's Hospital (US)
- Millennium Institute on Immunology and Immunotherapy (CL)
Publication Details
- Journal
- Clinical Epigenetics
- Published
- 2025-06-03
- DOI
- https://doi.org/10.1186/s13148-025-01889-x
- Citations
- 2
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Institutes of Health