lnc2300 mediates the induction of granulosa cell apoptosis by oxidative stress

lnc2300 (LOC102162300) is a newly characterized lncRNA that is down-regulated during sow follicular atresia and oxidative stress-stimulated sow granulosa cells (sGCs). However, the mechanism underlying its reduced abundance in atretic follicles and oxidative stress-stimulated sGCs remains unclear. Here, we reported that lnc2300 is transcriptionally suppressed by oxidative stress in a FoxO1-dependent manner. The lnc2300 promoter was characterized, and multiple <italic>cis</italic>-elements were identified, including the binding site of transcription factor FoxO1, an effector of oxidative stress. ChIP combined with reporter assays demonstrated that transcription factor FoxO1 directly binds to the lnc2300 promoter, thereby suppressing its activity. Furthermore, FoxO1 suppresses the lnc2300 transcript in sGCs by acting as a transcription repressor. Functionally, FoxO1 induces sGC apoptosis by suppressing the lnc2300 transcript. Additionally, FoxO1 mediated the promotion of oxidative stress on the lnc2300 transcript and its anti-apoptotic function in sGCs. Overall, these findings provide new insights into the mechanisms underlying the transcriptional regulation of lnc2300 and oxidative stress-induced sGC apoptosis.